It remains largely unknown where and how infections with soil-transmitted helminths (STHs; Ascaris, Trichuris, Necator and Ancylostoma) occur. We therefore aimed to identify possible sources of infection by assessing the environmental contamination in an STH-endemic area.
We first performed a series of laboratory experiments designed to optimize a soil straining-flotation method to detect and quantify Ascaris and Trichuris eggs in soil, and to validate the diagnostic performance of the optimized method when followed by microscopy and qPCR. In a second phase, we applied this method to assess the level of STH contamination in 399 environmental samples collected from 10 school compounds, 50 households and 9 open markets in Jimma Town (Ethiopia). Subsequently, we explored associations between the environmental contamination and both the corresponding STH epidemiology at the level of the schools and the household characteristics. Finally, we assessed the knowledge, attitude and practice (KAP) towards STHs in school children.
Our soil straining-flotation method has an analytical sensitivity of 50 eggs per 100 grams of soil and egg recovery rate of 36.0% (Ascaris) and 8.0% (Trichuris). The analysis of field samples with both microscopy and qPCR revealed the presence of 8 different helminth species of medical importance, including but not limited to the human STHs. There was a significant association between the environmental contamination and prevalence of any STH infections at the school level only. The KAP indicated a lack of knowledge and awareness of STHs.
Our optimized straining-flotation method has a moderate diagnostic performance and revealed that life stages of helminths are ubiquitous in the environment, which might be due to the poor sanitary facilities at both the schools and the households, and a poor level of KAP towards STHs. Further research is required to gain more insights into the contribution of these life stages to transmission.
How do IDPs and refugees fit within traditional, indigenous, and local knowledge of disasters?
Uneven displacement: How a village in coastal Guinea-Bissau is battling rising sea levels
Internal climate migration in a new era of scarcity
Ivar Kolstad, Päivi Lujala and Arne Wiig
EFFEXT Background Paper – National and international migration policy in Ethiopia
Kiya Gezahegne and Oliver Bakewell
Transoceania: Connecting the World beyond Eurasia.
Anthropology of Transformation: From Europe to Asia and Back
Building Back Better: The Politicisation of Disaster and Displacement Response Architecture in Lebanon
Re-imagining African—Nordic relations in a changing global order
Elling N. Tjønneland
Environmental justice for refugees in host countries: How Syrian refugees are disproportionately harmed by air and water pollution in Lebanon
John Hasan Yildiz
Qui détient quoi ? Trois leçons à tirer pour la transparence des bénéficiaires effectifs